Nanang Rahman, M.Pd, Nursina Sari


Tujuan penelitian ini adalah untuk mengembangkan buku petunjuk praktikum uji formalin dan  boraks dengan menggunakan indicator alami untuk siswa sekolah menegah pertama. Metode penelitian ini mengacu pada prosedur pengembangan dikembangkan oleh Borg & Galls. Uji coba terhadap 94 sampel daun dan mahkota bunga yang diperkirakan memiliki potensi sebagai indikator alami untuk uji formalin dan boraks telah dilakukan.  indikator alami yang dapat digunakan untuk uji tetes boraks, yaitu: bunga Ruellia Simplex, Curcuma Longa, Plumeria Rubra,dan Portulaca Grandiflora. Sedangkan untuk uji formalin dapat menggunakan Ruellia Ribosa, Hibiscus Rosa-Sinensis L dan Balsamina Impatiens L. Berdasarkan hasil penilaian ahli dan guru menunjukkan bahwa buku petunjuk praktikum telah layak digunakan untuk siswa Sekolah Menengah Pertama untuk menguji formalin dan boraks.


Pengembangan; Petunjuk Praktikum; Uji Formalin dan Borak

Full Text:



A. S. Irawan and L. S. Ani, “Prevalence Of Rhodamin B Contents, Formalin, And Boraks On Snack And The Description Of Knowledge Official Traders In Basic School Of Susut District, Bangli District,” vol. 5, no. 11, pp. 1–6, 2016.

X. Tang, Y. Bai, A. Duong, M. T. Smith, L. Li, and L. Zhang, “Formaldehyde in China: production, consumption, exposure levels, and health effects,” Environ. Int., vol. 35, no. 8, pp. 1210–1224, 2009.

P. A. Fail, R. E. Chapin, C. J. Price, and J. J. Heindel, “General, reproductive, developmental, and endocrine toxicity of boronated compounds,” Reprod. Toxicol., vol. 12, pp. 1–8, 1998.

D. Teshima et al., “Clinical management of boric acid ingestion: pharmacokinetic assessment of efficacy of hemodialysis for treatment of acute boric acid poisoning.,” J. Pharmacobiodyn., vol. 15, pp. 287–294, 1992.

EFSA, “Opinion of the Scientific Panel on Dietetic Products , Nutrition and Allergies on a request from the Commission related to the Tolerable Upper Intake Level of Boron ( Sodium Borate and Boric Acid ) ( Request N ° EFSA-Q-2003-018 ),” EFSA J., vol. 80, pp. 1–22, 2004.

Q. Li, M. Oshima, and S. Motomizu, “Flow-injection spectrofluorometric determination of trace amounts of formaldehyde in water after derivatization with acetoacetanilide,” Talanta, vol. 72, no. 5, pp. 1675–1680, 2007.

T. Katarzyna Różyło, A. Żabińska, and I. Różyło-Kalinowska, “Use of OPLC for quantitation of HCHO, as the dimedone adduct, in human saliva in different dental pathologies,” Jpc-Journal Planar Chromatogr. Tlc, vol. 15, no. 1, pp. 19–22, 2002.

B. A. Tomkins, J. M. McMahon, W. M. Caldwell, and D. L. Wilson, “Liquid chromatographic determination of total formaldehyde in drinking water.,” Journal-Association Off. Anal. Chem., vol. 72, no. 5, pp. 835–839, 1989.

V. Cogliano, Y. Grosse, R. Baan, K. Straif, B. Secretan, and F. El Ghissassi, “Advice on formaldehyde and glycol ethers,” Lancet Oncol., vol. 5, no. 9, p. 528, 2004.

S. Siti‐Mizura, E. S. Tee, and H. E. Ooi, “Determination of boric acid in foods: Comparative study of three methods,” J. Sci. Food Agric., vol. 55, no. 2, pp. 261–268, 1991.

L. M. Zeng, H. Y. Wang, and Y. L. Guo, “Fast Quantitative Analysis of Boric Acid by Gas Chromatography-Mass Spectrometry Coupled with a Simple and Selective Derivatization Reaction Using Triethanolamine,” J. Am. Soc. Mass Spectrom., vol. 21, pp. 482–485, 2010.

A. Hakim, A. Kadarohman, and Y. M. Syah, “Effects of the Natural Product Mini Project Laboratory on the Students Conceptual Understanding.,” J. Turkish Sci. Educ., vol. 13, no. 2, pp. 27–36, 2016.

K. M. Davies, “An introduction to plant pigments in biology and commerce,” Plant Pigment. their Manip., vol. 14, pp. 1–22, 2009.

K. M. Davies, “Modifying anthocyanin production in flowers,” in Anthocyanins, Springer, 2008, pp. 49–80.

R. Freyre, C. Uzdevenes, L. Gu, and K. H. Quesenberry, “Genetics and Anthocyanin Analysis of Flower Color in Mexican Petunia,” J. Am. Soc. Hortic. Sci., vol. 140, no. 1, pp. 45–49, 2015.

K. Torskangerpoll and Ø. M. Andersen, “Colour stability of anthocyanins in aqueous solutions at various pH values,” Food Chem., vol. 89, no. 3, pp. 427–440, 2005.

D. Burdulis, A. Sarkinas, I. Jasutiene, E. Stackeviciene, L. Nikolajevas, and V. Janulis, “Comparative Study of Anthocyanin Composition, Antimicrobial and Antioxidant Activity in Bilberry (Vaccinium Myrtillus L.) and Blueberry (Vaccinium Corymbosum L.) Fruits,” Acta Pol. Pharm., vol. 66, no. 4, pp. 399–408, 2009.

P. H. Março, R. J. Poppi, I. S. Scarminio, and R. Tauler, “Investigation of the pH effect and UV radiation on kinetic degradation of anthocyanin mixtures extracted from Hibiscus acetosella,” Food Chem., vol. 125, no. 3, pp. 1020–1027, 2011.

C. T. Du and F. J. Francis, “Anthocyanins of roselle (Hibiscus sabdariffa, L.),” J. Food Sci., vol. 38, no. 5, pp. 810–812, 1973.

Y. Nakamura, M. Hidaka, H. Masaki, H. Seto, and T. Uozumi, “Major anthocyanin of the flowers of Hibiscus (Hibiscus rosa-sinensis L.),” Agric. Biol. Chem., vol. 54, no. 12, pp. 3345–3346, 1990.

P. S. Vankar and D. Shukla, “Natural dyeing with anthocyanins from Hibiscus rosa sinensis flowers,” J. Appl. Polym. Sci., vol. 122, no. 5, pp. 3361–3368, 2011.

S. Clevenger, “The flavonols of Impatiens balsamina L,” Arch. Biochem. Biophys., vol. 76, no. 1, pp. 131–138, 1958.

J. Lee, R. W. Durst, and R. E. Wrolstad, “Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study,” J. AOAC Int., vol. 88, no. 5, pp. 1269–1278, 2005.

O. M. Andersen and K. R. Markham, Flavonoids: chemistry, biochemistry and applications. CRC press, 2005.

A. Gantait, T. Barman, and P. K. Mukherjee, “Validated method for estimation of curcumin in turmeric powder,” Indian J. Tradit. Knowl., vol. 10, no. 2, pp. 247–250, 2011.

W. Yj et al., “Stability of curcumin in buffer solutions and characterization of its degradation products . PubMed Commons,” J. Pharm. Biomed. Anal., vol. 15, no. 12, pp. 1867–1876, 1997.

R. Byamukama, J. Namukobe, M. Jordheim, Ø. M. Andersen, and B. T. Kiremire, “Scientia Horticulturae Anthocyanins from ornamental flowers of red frangipani , Plumeria rubra,” Sci. Hortic. (Amsterdam)., vol. 129, pp. 840–843, 2011.

D. Strack, T. Vogt, and W. Schliemann, “Recent advances in betalain research,” Phytochemistry, vol. 62, no. 3, pp. 247–269, 2003.

R. R. Leathers, C. Davin, and J. P. Zrÿd, “Betalain producing cell cultures ofBeta vulgaris L. var. bikores monogerm (red beet),” Vitr., vol. 28, no. 2, pp. 39–45, 1992.

G. Trejo-Tapia et al., “Influence of medium constituents on growth and betalain production in cell suspension cultures of Beta vulgaris,” Asia-Pacific J. Mol. Biol. Biotechnol., vol. 7, no. 2, pp. 167–172, 1999.

T. Akita, Y. Hina, and T. Nishi, “Production of betacyanins by a cell suspension culture of table beet (Beta vulgaris L.).,” Biosci. Biotechnol. Biochem., vol. 64, no. 9, pp. 1807–1812, 2000.



  • There are currently no refbacks.

Copyright (c) 2019 Jurnal Ulul Albab

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.