Kadar Flavonoid Total Daun Rhizopora Apiculata Blume Dengan Variasi Pelarut

Nurfijrin Ramadhani, Agung Giri Samudra, Wafa Syahidah, Cyntia Dwi Utami, Annisa Muslimah, Suci Rahmawati

Abstract


ABSTRAK

Rhizopora apiculata merupakan salah satu spesies mangrove yang tumbuh di pesisir pantai barat Bengkulu. Tanaman ini dilaporkan memiliki kandungan aktivitas antioksidan yang tinggi. Tujuan penelitian ini untuk mengetahui perbandingan kadar flavonoid dari daun Rhizopora apiculate menggunakan variasi pelarut metode maserasi dan sonikasi. Daun Rhizopora apiculate diekstraksi dengan metode maserasi selama 48 jam dan sonikasi selama 15 menit, masing-masing metode menggunakan pelarut heksan, etanol, metanol dan air. Ekstrak dilakukan skrining fitokimia dengan reaksi warna, kemudian dilanjutkan penetapan kadar total flavonoid pada ekstrak yang positif mengandung flavonoid menggunakan metode kolorimetri. Hasil kadar flavonoid dengan metode maserasi ekstrak, etanol, metanol dan air berturut turut adalah 8,503 ± 0,2917; 5,936 ± 0,182;  4,49 ± 0,095 mg EK/g ekstrak, sedangkan dengan metode sonikasi 6,736±0,271; 3,603 ± 0,22; 1,306 ±0,090 mg EK/g ekstrak. Berdasarkan hasil dapat disimpulkan kadar total flavonoid yang diperoleh paling tinggi pada ekstrak etanol dengan metode maserasi.

 

Kata kunci : Rhizopora apiculata; Pelarut; Maserasi; Sonikasi; Flavonoid


ABSTRACT

Rhizophora apiculata is one of the mangrove species that grows on the west coast of Bengkulu. This plant is reported to have a high content of an antioxidant activity. This study aimed to compare the flavonoid content of Rhizophora apiculata leaves using variations in solvents using maceration and sonication methods. Rhizophora apiculata leaves were extracted by the maceration method for 48 hours and sonication for 15 minutes, each using hexane, ethanol, methanol, and water as solvents. The extracts were screened for phytochemicals with a color reaction, then continued with the determination of total flavonoid levels in the positive flavonoid extracts using the colorimetric method. The results of flavonoid content by maceration method of extract, ethanol, methanol and water were 8.503 ± 0.2917; 5.936 ± 0.182; 4.49 ± 0.095 mg EK/g extract, while by sonication method 6.736±0.271; 3.603 ± 0.22; 1,306 ±0.090 mg EK/g extract. Based on the results, it can be concluded that the total flavonoid content obtained was the highest in the ethanol extract using the maceration method.

 

Keywords : Rhizopora apiculata; Solvents; Maseration; Sonication; Flavonoid.

 


Keywords


Rhizopora apiculata; Solvents; Maseration; Sonication; Flavonoid.

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References


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DOI: https://doi.org/10.31764/lf.v3i2.9328

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