Optimization of the Combination Ratio of Biduri and Bidara Leaf Extracts in Reducing Cholesterol in Mice

Jazilatur Rifqiyah, Cicik Wijayanti, Gaby Ayu Patricia, Siti Nur Husnul Yusmiati, Khoirul Ngibad

Abstract


This study aimed to evaluate the effectiveness of a combination of ethanol extracts of Biduri (Calotropis gigantea L.) and Bidara (Ziziphus spina-christi L.) leaves in reducing cholesterol levels in mice induced with hypercholesterolemia, as well as to determine the optimal dose and combination ratio. This experimental laboratory study involved several stages, including extract preparation, preparation of extract suspensions, preparation of simvastatin and propylthiouracil (PTU) suspensions, selection and maintenance of experimental animals, administration of treatments, and measurement of blood cholesterol levels. A total of 72 male mice were divided into 12 treatment groups: negative control, positive control, drug control, and nine groups receiving combinations of extracts with ratios of 1:1, 1:2, and 2:1 at three doses (14, 28, and 56 mg/20 g BW). Hypercholesterolemia was induced using PTU for 14 days, followed by oral administration of the extract combinations for 3 days. Cholesterol levels were measured using a spectrophotometer and analyzed using One Way ANOVA. The results showed that the combination of Biduri and Bidara extracts significantly reduced cholesterol levels (p < 0.05), with the highest reduction observed in the 2:1 ratio at a dose of 56 mg/20 g BW, showing a 59.18% decrease (135.47 mg/dL) compared to the positive control. These findings indicate a synergistic effect between the two extracts and suggest their potential as a natural hypocholesterolemic agent.


Keywords


Biduri; Bidara; Hypercholesterolemia; Mice

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References


Amin, S., Nasywa, P., Ma, N., Permatasari, I., & Maryam, S. (2025). Exploration of Medicinal Chemistry for High Cholesterol Therapy: Structure–Activity Relationship Analysis and Molecular Drug Targets. 4(April).

Ayalew, M., & Bekele, A. (2021). Evaluation of the Antimalarial Activity of Crude Extract of Eucalyptus globulus Labill . Leaf against Plasmodium berghei in Mice. Hindawi, 9. https://doi.org/10.1155/2021/7068999

Kallapur, A., & Sallam, T. (2023). Trends in Cardiovascular Medicine Pharmacotherapy in familial hypercholesterolemia - Current state and emerging paradigms. Trends in Cardiovascular Medicine, 33(3), 170–179. https://doi.org/10.1016/j.tcm.2021.12.011

Kaundal, R., & Kumar, D. (2025). Medicine in Drug Discovery Current demands for standardization of Indian medicinal plants : A critical review. Medicine in Drug Discovery, 27(May), 100211. https://doi.org/10.1016/j.medidd.2025.100211

Lailatusholihah, I., Setyoko, L. P., Wijayanti, S., Situmeang, B., Musa, W. J., & Bialangi, N. (2023). Cholesterol-Lowering Activity of n-Hexane Extract of Bidara Leaves (Ziziphus mauritiana). CHEDS: Journal of Chemistry, Education, and Science, 7(2), 232–237. https://doi.org/10.30743/cheds.v7i2.8398

Laka, K., Makgoo, L., & Mbita, Z. (2022). Cholesterol-Lowering Phytochemicals : Targeting the Mevalonate Pathway for Anticancer Interventions. 13(March), 1–22. https://doi.org/10.3389/fgene.2022.841639

Lohvina, H., Sándor, M., & Wink, M. (2021). Effect of EthanolSolvents on Total Phenolic Content and AntioxidantProperties of Seed Extracts of Fenugreek (Trigonella foenum-graecum L.) Varieties and Determination of Phenolic Composition by HPLC-ESI-MS. Diversity, 1(14), 7.

Maulidya, V., Eka, Y., Silvany, E., Dwi, M., Zahratul, H., Shafa, M., Klau, I. C. S., Farmasi, S., Kesehatan, F. I., & Medika, U. A. (2025). Review Article: Ethnobotanical Exploration of Medicinal Plants for Traditional Medicine among the Tengger Tribe in Lumajang.

Maynita, S., Pgri, U., Pujiati, M., Weka, M., Bhagawan, S., Cicilia, M., & Primiani, N. (2023). Analysis of the Yield of Ethanol Extract from Genitri Leaves from Semarang. Seminar Nasional Prodi Farmasi UNIPMA (SNAPFARMA), 2023, 162–167. http://prosiding.unipma.ac.id/index.php/SNAPFARMA

Mitar, A., & Kardum, J. P. (2020). Intensification of Mass Transfer in the Extraction Process with a Nanofluid Prepared in a Natural Deep Eutectic Solvent. Chemical Engineering & Technology, 43(11), 2286–2294.

Munandar, K. A., Pradana, M. S., & Ngibad, K. (2025). Effect of a Combination of Biduri Leaf Extract and Saga Leaf Extract on the Reduction of Cholesterol Levels in Hypercholesterolemic Mice. 8(2), 174–180.

Ngibad, K., Nuryani, E. D., Mukarromah, T. S., & Fadhila, N. (2025). Antihypercholesterolemic Potential of Ethanolic Leaf Extracts of Santalum album L ., Calotropis gigantea , Ziziphus spina -christi. 11(6), 846–852. https://doi.org/10.29303/jppipa.v11i6.11499

Riyadina, W. (2019). Hypertension in Menopausal Women. LIPI Press.

Rossini, E., Biscetti, F., Rando, M. M., Nardella, E., Cecchini, A. L., Nicolazzi, M. A., Covino, M., Gasbarrini, A., Massetti, M., & Flex, A. (2022). Statins in High Cardiovascular Risk Patients : Do Comorbidities and Characteristics Matter ? risk category and LDL target Age and Comorbidities Drug and and possible adverse LDL-C B-containing.

Safitri, A. F., Tanggasari, D., Studi, P., Industri, T., Pertanian, F. T., & Teknologi, U. (2022). Analysis of the Physicochemical Characteristics of Bidara Leaves (Ziziphus spina-christi L.). yang dikeringkan Menggunakan Tray Dryer dengan Suhu Berbeda. 2(1), 49–58.

Sanz, J. L., Soler-Doria, A., López-García, S., García-Bernal, D., Rodríguez-Lozano, F. J., Lozano, A., Llena, C., Forner, L., Guerrero-Gironés, J., & Melo, M. (2021). ComparativeBiological Properties and Mineralization Potential of 3 Endodontic Materials for Vital Pulp Therapy: Theracal PT, Theracal LC, and Biodentine on Human Dental Pulp StemCells. Journal of Endodontics, 47(12), 1896–1906.

Shivanandappa, T. B., Chinnadhurai, M., & Kandasamy, G. (2023). Ziziphus mauritiana Leaves Normalize Hormonal Profile and Total Cholesterol in Polycystic Ovarian Syndrome Rats.

World Health Organization (WHO). (2025). Penyakit kardiovaskular (PKV). https://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds)

Yudha, A. K., & Suidah, H. (2023). Correlation Study of Dietary Patterns and Cholesterol Levels in Stroke Patients . JURNAL PENGEMBANGAN ILMU DAN PRAKTIK KESEHATAN, 2(1), 48–61.

Yudha, N. S. D., Arsana, P. M., & Rulli, R. (2021). Comparison of Lipid Profiles in Patients with Type 2 Diabetes Mellitus with Good Glycemic Control and Poor Glycemic Control in Perbandingan Profil Lipid pada Pasien Diabetes Melitus Tipe 2 dengan Kontrol Glikemik yang Terkendali dan Kontrol Glikemik yang. 8(4). https://doi.org/10.7454/jpdi.v8i4.592

Zhang, X., Chen, J., Yi, K., Peng, L., Xie, J., Gou, X., Peng, T., & Tang, L. (2020). Phlorizin ameliorates obesity-associated endotoxemia and insulin resistance in high-fat diet-fed mice by targeting the gut microbiota and intestinal barrier integrity. Gut Microbes, 12(1), 1–18. https://doi.org/10.1080/19490976.2020.1842990




DOI: https://doi.org/10.31764/justek.v8i4.36437

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