ISOLASI LIKOPEN DARI BUAH TOMAT (LYCOPERSICUM ESCULENTUM) DENGAN PELARUT HEKSANA
Abstract
Penelitian ini bertujuan untuk mengembangkan metode sederhana dan efektif dengan menggunakan ekstraksi pelarut heksana dan methanol sebagai anti pelarut untuk isolasi likopen dari buah tomat (Lycopersicum esculentum). Tomat merupakan sumber utama likopen. Likopen adalah zat pigmen golongan karotenoid yang menyebabkan warna merah pada tomat. Penelitian ini telah berhasil mengisolasi likopen dengan metode maserasi. Hasil penelitian menunjukkan dengan pelarut heksana kadar likopen yang diperoleh 2,25 mg/100 g. Analisa gugus fungsi dengan menggunakan spektroskopi Fourier Transform Infra Red (FT-IR) terdeteksi gugus C=C pada panjang gelombang 1537,09 cm-1, gugus CH2 terdeteksi pada panjang gelombang 1498,86 cm-1, gugus R-CH=CH-R terdeteksi pada panjang gelombang 959,27 cm-1. Sedangkan gugus regangan C-C dan C-C-H (streching)masing-masing terjadi pada panjang gelombang 1262,15 cm-1 dan 1378,71 cm-1.
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DOI: https://doi.org/10.31764/agrotek.v5i2.707
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