Maximizing the Extraction of Albumin from Common Carp (Cyprinus carpio Linnaeus, 1758) with Variations in Temperature and HCl Solvent Concentrations

Nurfaidah nurfaidah

Abstract


Albumin is a globular protein that plays a crucial role in various biological functions, including regulating blood osmotic pressure and transporting small molecules in the body. With the growing demand and high production costs of albumin derived from human plasma, exploring alternative sources such as fish is important. This study aims to optimize the extraction of albumin from common carp (Cyprinus carpio) using hydrochloric acid (HCl) solvent at different concentrations and heating temperatures. The fish flesh, after being finely ground, was mixed with HCl solvent and heated in a water bath for 30 minutes. The results showed that albumin yields varied between 0.94% and 3.55% (w/w). The highest yield was achieved with 0.1 M HCl at 35°C, reaching 3.55%, while the lowest yield was obtained with 0.2 M HCl at 27°C, which was 0.94%. Moderate temperatures and the appropriate HCl concentration allow effective protein denaturation without excessive damage, making it the optimal extraction method. These findings indicate that extraction with 0.1 M HCl at 35°C is the best condition for maximizing albumin yield from common carp. The study also provides a basis for further exploration of alternative extraction methods and environmentally friendly solvents, as well as an economic feasibility assessment at the industrial scale.

Keywords


Albumin; Extraction; HCl; Yield

References


Andoyo, R., Diani, A.F.F. 2023. Effect of heating temperature on physical , functional , and digestibility properties of Whey Protein Concentrate (WPC). IOP Conference Series: Earth and Enviromental Science. IOP Publishing. https://doi.org/10.1088/1755-1315/1230/1/012155

Andreeva, A.M. 2022. Evolutionary transformations of albumin using the example of model species of jawless agnatha and bony jawed fish (Review). Inland Water Biology 15(5): 641–658. https://doi.org/10.1134/S1995082922050029

Asfar, M., Tawali, A.B., Abdullah, N., Mahendradatta, M. 2014. Extraction of albumin of snakehead fish (Channa striatus) in producing the fish protein concentrate (FPC). International Journal of Scientific & Technology Research 3(4): 85–88.

Asfar, M., Tawali, A.B., Mahendradatta, M. 2019. Extraction of albumin of a snakehead fish (Channa striata) at its isoelectric point. Agercolere 1(1): 6–12.

Asikin, A. N., Kusumaningrum, I. 2018. Albumin profile of snakehead fish (Channastriata) from East Kalimantan, Indonesia. IOP Conference Series: Earth and Environmental Science, 144(1). IOP Publishing. https://doi.org/10.1088/1755-1315/144/1/012035

Belinskaia, Daria A., Polina A. Voronina, Anastasia A. Batalova, Nikolay V. Goncharov. 2021. Serum Albumin. Encyclopedia 1(1): 65-75. https://doi.org/10.3390/encyclopedia1010009

Bhokarikar, S., Poojitha, P., Vijay, V., Adithya, S. P., Gurumoorthi, Kapoor, A. 2024. Parameters affecting the efficiency of extraction systems in the food industries. In: Extraction processes in the food industry, Woodhead Publishing, pp. 397–434. https://doi.org/10.1016/B978-0-12-819516-1.00010-7

Chasanah, E., Susilowati, R., Yuwono, P., Zilda, D.S., Fawzya, Y.N. 2019. Amino acid profile of biologically processed fish protein hydrolysate (FPH) using local enzyme to combat stunting. IOP Conference Series: Earth and Environmental Science, 278(1). IOP Publishing. https://doi.org/10.1088/1755-1315/278/1/012013

Firlianty. 2016. Vacuum drying albumin powder of snakehead (Channa micropeltes) potential for wound healing from Central Kalimantan, Indonesia. International Journal of ChemTech Research 9(5): 263–269.

Fitriyani, E., Nuraenah, N. 2023. Chemical, physical, and hedonic quality characteristics of fish cereal from toman fish albumin extract (Channa micropeltes) as functional food. International Journal of Multi Discipline Science 6(2): 142–155.

Foegeding, E.A., Allen, C.E., Dayton, W.R. 1986. Effect of heating rate on thermally formed myosin, fibrinogen and albumin gels. Journal of Food Science 51(1): 104–108. https://doi.org/10.1111/j.1365-2621.1986.tb10846.x

Hariati, A.M., Yuniarti, A., Endariani, Kusuma, W.E., Wiadnya, D.G.R. 2019. Albumin and enzyme profiles of dwarf snakehead, Channa gachua caught from River Brantas, East Java. Journal of Physics: Conference Series 1146(1). https://doi.org/10.1088/1742-6596/1146/1/012041

Hutapea, T.P.H., Madurani, K.A., Syahputra, M.Y., Hudha, M.N., Asriana, A.N., Suprapto, Kurniawan, F. 2023. Albumin: Source, preparation, determination, applications, and prospects. Journal of Science: Advanced Materials and Devices 8(2): 100549. https://doi.org/10.1016/j.jsamd.2023.100549

Hutapea, T.P.H., Suprapto, Kurniawan, F. 2022. Extraction of dissolved protein of Channa striata from Tarakan, North Borneo as a source of fish serum albumin. IOP Conference Series: Earth and Environmental Science, 1083(1). IOP Publishing. https://doi.org/10.1088/1755-1315/1083/1/012080

Fortune Business Insight 2024. Albumin Market Size, Share & Industry Analysis, By Product (Human Serum Albumin, Recombinant Albumin, and Bovine Serum Albumin), By Application (Therapeutics, Diagnostics, and Research), By End-user (Hospital & Clinics, Pharmaceutical & Biotechnology Industry, and Research Institutes), and Regional Forecast, 2024-2032. https://www.fortunebusinessinsights.com/albumin-market-102637. (Accessed 31 August, 2024)

Jamaluddin, Hasnawati, Yuyun, Y., Pitriani, Widodo, A. 2021. Effect of extraction method of the eel fish albumin levels of Anguilla marmorata (Q.) gaimard and Anguilla bicolor. Journal of Physics: Conference Series 1763(1). https://doi.org/10.1088/1742-6596/1763/1/012080

Meena, C.S., Kumar, A., Roy, S., Cannavale, A., Ghosh, A. 2022. Review on boiling heat transfer enhancement techniques. Energies 15: 5759. https://doi.org/ 10.3390/en15155759

Nugroho, M. 2013. The effect of temperature and duration of the steaming extraction albumin content and yield from the fish gabus (Ophiocephalus striatus). Indonesian Journal of Fisheries Science and Technology 9(1): 40–48.

Nurfaidah, Metusalach, Sukarno, Mahendradatta, M. 2021. Protein and albumin contents in several freshwater fish species of Makassar, South Sulawesi, Indonesia. International Food Research Journal 28(4): 745–751. https://doi.org/10.47836/ifrj.28.4.11

Pioro, I.L. 2024. Advances and challenges of boiling heat transfer. In: advances in boiling and condensation transform. IntechOpen. http://dx.doi.org/10.5772/intechopen.114095

Qixing, J., Zhengran, M., Shuoshuo, W., Yanshun, X., Fengyu, T., Xueqin, X., Peipei, Y., Wenshui, X. 2014. Effect of temperature on protein compositional changes of big head carp (Aristichthys nobilis) muscle and exudates. Food Science and Technology Research 20(3): 655–661. https://doi.org/10.3136/fstr.20.655

Rashidi, S., Hormozi, F., Mohsen, M. 2020. Potentials of boiling heat transfer in advanced thermal energy systems. Journal of Thermal Analysis and Calorimetry 143(3). https://doi.org/10.1007/s10973-020-09511-3

Romadhoni, A.R., Afrianto, E., Pratama, R.I., Grandiosa, R. 2016. Extraction of snakehead Ffsh [Ophiocephalus Striatus (Bloch, 1793)] into fish protein concentrate as albumin source using various solvent. Aquatic Procedia 7: 4–11. https://doi.org/10.1016/j.aqpro.2016.07.001

Santoso, H. 2019. Analysis of albumin levels in farmed sidat fish (Anguilla marmorata) and natural breed toman fish (Channa Micropeltes). In Proceedings of BROMO Conference-Symposium on Natural Product and Biodiversity, pp 288–291. https://doi.org/10.5220/0008361502880291

Setiawan, H., Intanon, M., Chummitri, P., Sringarm, K., Sathanawongs, A. 2024. Snakehead fish, Channa striata (bloch, 1973), protein concentrate: excellent recovery of fish-based albumin source and its possible application for sperm capacitation. Asian Fisheries Science 37(2): 125–137. https://doi.org/10.33997/j.afs.2024.37.2.005

Setyawati, O., Putra, A.P., Nurussa'adah. 2024. Temperature control system for albumin extraction and pasteurization process of snakehead-fish (Channa striata). In: BIO Web of Conferences 92, 02018.

Susilowati, R., Januar, H.I., Fithriani, D., Chasanah, E. 2015. The potency of aquaculture freshwater fishes as a raw material for fish serum albumin based nutraceutical products. Journal of Postharvest and Marine and Fisheries Biotechnology 10(1): 37. https://doi.org/10.15578/jpbkp.v10i1.243

Susilowati, R., Sugiyono, S., Chasanah, E. 2016. Nutritional and albumin content of swamp fishes from Merauke, Papua, Indonesia. Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology 11(3): 107-116. https://doi.org/10.15578/squalen.v11i3.268

Suwandi, R., Winem, M. 2014. Body parts proportion and proximate levels of snakehead on various sizes. Indonesian Journal of Aquatic Product Technology 17(1): 22–28.

The Lancet Haematology. 2017. The big business of blood plasma. The Lancet Haematology, 4(10), e452. https://doi.org/10.1016/S2352-3026(17)30183-7

Tsai, W., Chiu, Y., Wu, H., Sun, W. 2021. Boiling for 15 minutes improves nutrient profiles in fishes included in the diets of dialysis patients. Hemodialysis International 4: 541-547. https://doi.org/10.1111/hdi.12947

Soedjanaatmadja, U.M.S., Nadiya, A., Wildan, A., Saadah, D.R., Shabarni, G., Safri, I., Ari, H. 2021. Albumin, as a therapeutic protein: potential source, application, isolation and purification. International Journal of Research in Pharmaceutical Sciences 12(3): 1922–1931. https://doi.org/10.26452/ijrps.v12i3.4794

Vujadinović, D., Grujić, R., Tomović, V., Torbica, A. 2014. Effects of temperature and method of heat treatment on myofibrillar proteins of pork. Chemical Industry and Chemical Engineering Quarterly 20(3): 407–415. https://doi.org/10.2298/CICEQ121011023V

Wirahadikusumah, M. 1981. Biokimia: Protein, Enzim dan Asam Nukleat. ITB Bandung.

Wiranata, N., Baehaki, A., Lestari, S. 2015. Pengaruh rasio etanol dan air cucian surimi ikan gabus (Channa striata) terhadap recovery protein larut air. FishtecH 4(1), 1–8.




DOI: https://doi.org/10.31764/jafp.v5i1.30097

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