Antibacterial activity of herbal tea extract from lotus leaves (Nelumbo nucifera) using different solvents

Muhammad Syifa; Rita Khairina; Findya Puspitasari

doi:10.31764/jau.v11i2.22060

Antibacterial activity of herbal tea extract from lotus leaves (Nelumbo nucifera) using different solvents

Muhammad Syifa, Rita Khairina, Findya Puspitasari

Abstract

This research aimed to examine the antibacterial activity of lotus leaf (Nelumbo nucifera) extract as a basis for herbal tea production. Lotus leaf was chosen as the herbal tea ingredient due to its abundant availability in South Kalimantan and its believed beneficial phytochemical content for health. The research process involved several stages including lotus leaf herbal tea processing, multi-stage maceration extraction using hexane, ethyl acetate, and ethanol solvents, and testing the extract's ability against Escherichia coli bacteria. The research design employed a 2-factor complete randomized design, with the first factor being the extract concentration and the second being the solvent type. Data analysis was conducted using Two-Way ANOVA in IBM SPSS 25. The results revealed that ethanol solvent produced the highest concentration extract with a yield of 10.86%, while hexane solvent yielded the lowest at 1.64%. Subsequent antibacterial activity testing was carried out using direct contact method with lotus leaf extract concentrations of 0.5%, 1%, and 1.5% in all three solvents. The findings indicated that 0.5% and 1% concentration extracts inhibited Escherichia coli growth across all solvents, whereas the 1.5% extract concentration in ethanol was capable of killing Escherichia coli bacteria. It was concluded that lotus leaf extract holds potential as an antibacterial agent, with higher extract concentrations showing greater efficacy in inhibiting bacterial growth. The 1.5% concentration (45 μl) exhibited the most effective results in killing Escherichia coli, underscoring the potential of Nelumbo nucifera lotus leaf in the development of antibacterial herbal products.

Keywords

antibacterial; herbal tea; lotus leaf; organic solvent

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References

Aksara, R., Musa, W. J. A., & Alio, L. (2013). Identifikasi Senyawa Alkaloid Dari Ekstrak Metanol Kulit Batang Mangga (Mangifera indica L). Jurnal Entropi, 8(1), 514–519. https://repository.ung.ac.id/get/simlit_res/1/477/Identifikasi-Senyawa-Alkaloid-Dari-Ekstrak-Metanol-Kulit-Batang-Mangga-Mangifera-indica-L-Penulis2.pdf

Anggraini, W., Nisa, S. C., DA, R. R., & ZA, B. M. (2019). Aktivitas Antibakteri Ekstrak Etanol 96% Buah Blewah (Cucumis melo L. var. cantalupensis) terhadap pertumbuhan bakteri Escherichia coli. Pharmaceutical Journal of Indonesia, 5(1), 61–66.

Badaring, D. R., Sari, S. P. M., Nurhabiba, S., Wulan, W., & Lembang, S. A. R. (2020). Uji Ekstrak Daun Maja (Aegle marmelos L.) terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus. INDONESIAN JOURNAL OF FUNDAMENTAL SCIENCES (IJFS), 6(01), 16–26.

Capasso, R., Evidente, A., Schivo, L., Orru, G., Marcialis, M. A., & Cristinzio, G. (1995). Antibacterial Polyphenols From Olive Oil Mill Waste Waters. Journal of Applied Bacteriology, 79(4), 393–398. https://doi.org/10.1111/j.1365-2672.1995.tb03153.x

Chandra, B., Asra, R., & Mevia, N. A. (2022). Perbedaan Ekstraksi Daun Teratai (Nymphaea Pubescens Willd) Sebagai Fungsi Aktivitas Antioksidan. Jurnal Farmasi Higea, 14(1), 28. https://doi.org/10.52689/higea.v14i1.434

De, C. L. (2020). Indian Lotus -A Multipurpose Aquatic Ornamental Plant. Vigyan Varta, 1(5), 7–9.

Dewata, I. P., Wipradnyadewi, P. A. S., & Widarta, I. W. R. (2017). Antioksidan Dan Sifat Sensoris Teh Herbal Daun Alpukat. Itepa, 6(2), 30–39.

Dewi, B. A., Wardani, T. S., & Nurhayati, N. (2021). Fitokimia (2021st ed.). PUSTAKABARUPRESS.

Diponegoro, N. M. (2022). Kandungan Fitokimia Teh Herbal Daun Lotus (Nelumbo nucifera). In digilib.ulm.ac.id. Universitas Lambung Mangkurat.

Diputra, P. M. A. S., Yusasrini, N. L. A., & Permana, I. D. G. M. (2023). Pengaruh Tingkat Ketuaan Daun terhadap Karakteristik Teh Herbal Daun Sirsak (Annona muricata Linn.). Itepa: Jurnal Ilmu Dan Teknologi, 12(2), 250–263.

Fitrial, Y., & Khotimah, I. K. (2017). Antibacterial Activity of Melanin from Cuttlefish and Squid Ink. Jurnal Pengolahan Hasil Perikanan Indonesia, 20(2), 266–274. https://doi.org/10.17844/jphpi.v20i2.17907

Hari Triandini, I. G. A. A., Wangiyana, I. G. A. S., Ratnaningsih, Y., & Rita, R. R. N. D. (2022). Pelatihan Pembuatan Teh Herbal Penunjang Primary Health Care Selama Masa Pandemi Covid-19 Bagi Ibu Pkk Tanjung Karang Kota Mataram. SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan, 6(2), 630. https://doi.org/10.31764/jpmb.v6i2.8315

Karmilah, Reymon, Nur Saadah Daud, Esti Badia, Agung Wibawa Mahatva Yodha, Muh. Azdar Setiawan, Selfyana Austin Tee, & Musdalipah. (2023). Aktivitas Antibakteri Rimpang Meistera chinensis terhadap Bakteri Staphylococcus aureus ATCC 25023 dan Eschericia coli ATCC 35218 Secara Difusi Agar. Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati, 8(1), 10–18. https://doi.org/10.24002/biota.v8i1.5651

Kemit, N., Widarta, I. W. R., & Nocianitri, K. A. (2016). Pengaruh Jenis Pelarut dan Waktu Maserasi Terhadap Kandungan Senyawa Flavonoid dan Aktivitas Antioksidan Ekstrak Daun Alpukat ( Persea Americana Mill ). Jurnal Ilmu Teknologi Pangan, 5(2), 130–141.

Kusumaningrum, R., Supriadi, A., & Hanggita, S. (2013). KARAKTERISTIK DAN MUTU TEH BUNGA LOTUS (Nelumbo nucifera). FishtecH – Jurnal Teknologi Hasil Perikanan, 2(1).

Mahmudah, D. A. (2022). Uji Aktivitas Antibakteri Teh Herbal Bunga Lotus (Nelumbo nucifera) terhadap Bakteri Escherichia Coli. In digilib.ulm.ac.id. Universitas Lambung Mangkurat.

Martini, N. K. A., Ekawati, I. A., & Ina, P. T. (2020). Pengaruh Suhu dan Lama Pengeringan terhadap Karakteristik Teh Bunga Telang (Clitoria ternatea L.). Jurnal Itepa, 9(3), 327–340.

Maryam, S., Rahmawati, & Abidin, Z. (2022). Pembuatan Minuman Herbal Antioksidan sebagai Peningkat Sistem Imun untuk Mencegah Infeksi Covid-19. Indonesian Journal of Community Dedication (IJCD), 4(2), 32–39.

Nurjanah, Hidayat, T., & Abdullah, A. (2019). Pengetahuan Bahan Baku Industri Hasil Perairan: Penuntun Praktikum. Bogor: IPB Press (Issue March). IPB Press.

Pangestu, N. (2022). Kandungan Fitokimia Teh Herbal Bunga Lotus (Nelumbo nucifera). In digilib.ulm.ac.id. Lambung Mangkurat.

Permadi, A., Sutanto, & Wardatun, S. (2015). Perbandingan Metode Ekstraksi Bertingkat dan Tidak Bertingkat terhadap Flavonoid Total Herba Ciplukan (Physalis angulata L.) secara Kolorimetri. Jurnal Online Mahasiswa (JOM) Bidang Farmasi, 1(1), 1–10.

Prayoga, E. (2013). Perbandingan Efek Ekstrak Daun Sirih Hijau (Piper betle L.) dengan Metode Difusi Disk dan Sumuran terhadap Pertumbuhan Bakteri Staphylococcus aureus. In Universitas Islam Negeri Syarif Hidayatullah. Universitas Islam Negeri Syarif Hidayatullah.

Putri, W. S., Warditiani, N. K., & Larasanty, L. P. F. (2018). Skrining Fitokimia Ekstrak Etil Asetat Kulit Buah Manggis (Garcinia mangostana L.). Jurnal Farmasi Udayana, 2(4), 56–60.

Putri, Y. S., Wangiyana, I. G. A. S., & Nahlunnisa, H. (2021). Jurnal Silva samalas. Jurnal Silva Samalas Journal of Forestry and Plant Science, 4(2), 39–44.

Rahmawatiani, A., Mayasari, D., & Narsa, A. C. (2020). Kajian Literatur: Aktivitas Antibakteri Ekstrak Herba Suruhan (Peperomia pellucida L.). Proceeding of Mulawarman Pharmaceuticals Conferences, 12(April 2021), 117–124. https://doi.org/10.25026/mpc.v12i1.401

Reiza, I. A., Rijai, L., & Mahmudah, F. (2019). Skrining Fitokimia Ekstrak Etanol Kulit Nanas (Ananas comosus (L.) Merr). Proceeding of Mulawarman Pharmaceuticals Conferences, 10(April 2021), 104–108. https://doi.org/10.25026/mpc.v10i1.371

Ridhowati, S., Kriska, Rachmawati, S. H., Sari, D. I., & Lestari, S. (2023). Pemanfaatan Daun Bunga Lotus (Nelumbo nucifera) Sebagai Minuman Herbal. Journal Perikanan, 13(3), 825–836.

Romadanu, R., Hanggita, S., & Lestari, S. D. (2014). PENGUJIAN AKTIVITAS ANTIOKSIDAN EKSTRAK BUNGA LOTUS (Nelumbo nucifera). Jurnal FishtecH, 3(1), 1–7. https://doi.org/10.36706/fishtech.v3i1.3523

Saptowo, A., Supriningrum, R., & Supomo. (2022). UJI AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BATANG SEKILANG (Embeliaborneensis Scheff) TERHADAP BAKTERI Propionibacterium acnes dan Staphylococcus epidermidis. Al-Ulum: Jurnal Sains Dan Teknologi, 7(2), 93–97. https://doi.org/10.31602/ajst.v7i2.6331

Senduk, T. W., Montolalu, L. A. D. Y., & Dotulong, V. (2020). Rendemen Ekstrak Air Rebusan Daun Tua Mangrove Sonneratia alba. Jurnal Perikanan Dan Kelautan Tropis, 11(1), 9. https://doi.org/10.35800/jpkt.11.1.2020.28659

Sharan, M., & Haldar, S. (2021). Lotus (Nelumbo nucifera) - An Exploration of Hygro waste for Textile Applications. Acta Scientific Agriculture, 5(5), 119–126. https://doi.org/10.31080/asag.2021.05.0997

Syafitri, N. E., Bintang, M., & Falah, S. (2014). Kandungan Fitokimia , Total Fenol , dan Total Flavonoid Ekstrak Buah Harendong (Melastoma affine D. Don). Kandungan Fitokimia, Total Fenol, Dan Total Flavonoid Ekstrak Buah Harendong, 1(3), 105–115.

Wendersteyt, N. V., Wewengkang, D. S., & Abdullah, S. S. (2021). Uji Aktivitas Antimikroba dari Ekstrak dan Fraksi Ascidian Herdmania Momus dari Perairan Pulau Bangka Likupang terhadap Pertumbuhan Mikroba Staphylococcus aureus, Salmonella typhimurium dan Candida albicans. Pharmacon, 10(1), 706–712. https://doi.org/10.35799/pha.10.2021.32758

DOI: https://doi.org/10.31764/jau.v11i2.22060