The Effect of the Combination of 2,4-D and BAP and Lighting Conditions on Callus Induction of Sugarcane (Saccharum officinarum L.) Variety CMG Agribun

Lailatun Ni'mah, Noor Aini Habibah

Abstract


This study aimed to evaluate the effect of different combinations of 2,4-D and BAP, as well as light conditions, on callus induction of sugarcane variety ‘CMG Agribun’. A factorial Randomized Complete Block Design (RCBD) was applied with two factors: plant growth regulators (PGRs) combinations (2,4-D and BAP) and lighting conditions (light and dark). Observed parameters included callus initiation time, percentage of callus formation, fresh and dry weight, and callus morphology. The results showed that the treatments significantly affected callus initiation time but not the percentage of callus formation. The fastest callus induction (2.83 DAP) occurred under dark conditions with 1 ppm 2,4-D + 0 ppm BAP and 4 ppm 2,4-D + 1 ppm BAP. The fresh weight of the callus did not show any significant differences among the treatments, but the treatment under dark conditions tended to produce a higher fresh weight of the callus. The highest dry weight (0.092 g) was also obtained from the treatment 1 ppm 2,4-D + 0.5 ppm BAP under dark conditions. Callus morphology was generally compact with varying colors. Further research is recommended using a wider range of 2,4-D and BAP concentrations, as well as different light regimes, to optimize callus induction and development. This study supports further research on callus-based propagation and the production of secondary metabolites in sugarcane.


Keywords


Callus Induction; Sugarcane; 2,4-D; BAP; Lighting.

Full Text:

PDF

References


Abdullah, D., Lestari, A., & Suhesti, S. (2023). Response of Callus Induction in Sugarcane Plant (Saccharum officinarum L.) ASA Agribun Variety to Various Concentrations Of 2,4-D (Dichlorophenoxyacetic acid) and BAP (Benzylaminopurine). Jurnal Agrotech 8(1), 120-126. https://doi.org/10.31970/agrotech.v13i2.145

Ali, S. E., El Gedaily, R. A., Mocan, A., Farag, M. A., & El-Seedi, H. R. (2019). Profiling Metabolites and Biological Activities of Sugarcane (Saccharum officinarum Linn.) Juice and Its Product Molasses Via a Multiplex Metabolomics Approach. Molecules, 24(5). https://doi.org/10.3390/molecules24050934

Arsyam, A., Abdullah, A., & Syam, N. (2020). Regeneration Power of The Embryo Explants Callus Soybean (Glycine max L.) at Various Concentrations Growth Hormones of 2.4 D And BAP In Vitro. AGrotekMAS Jurnal Indonesia: Jurnal Ilmu Pertanian, 1(3). https://doi.org/10.33096/agrotekmas.v1i3.113

Basri, A. H. H. (2016). Study on the Use of Tissue Culture for the Propagation of Virus-Free Plants. Agrica Ekstensia, 10(1), 64-73. https://doi.org/10.31764/jmm.v4i5.3049

Busaifi, R. (2018). Induction of Embryogenic Callus in Sugarcane (Saccharum officinarum L.) in Various Combinations of 2.4D and BAP Through in Vitro. AGROSAINS 5(2). https://doi.org/10.31102/agrosains.2018.5.2.91-102

Fatahillah, R., Rahmi, H., Saputro, N. W., & Suhesti, S. (2024). The Effect of a Combination of 2,4-D (Dichlorophenoxyacetic acid) and BAP (Benzyl Amino Purine) on MS (Murashige and Skoog) Medium on Callus Induction in Sugarcane (*Saccharum officinarum* L.) Variety AAS Agribun. Jurnal Agroplasma 11(1). https://doi.org/10.36987/agroplasma.v11i1.5412

Fu, R., Deng, Q., Wang, J., Li, Y., Xu, L., Tang, G., Li, W., Yu, X., & Xiang, L. (2025). Induction and Transformation of Friable Callus in Chrysanthemum ‘Jimba.’ Horticulturae, 11(10). https://doi.org/10.3390/horticulturae11101267

Han, J., Li, Y., Zhao, Y., Sun, Y., Li, Y., & Peng, Z. (2024). How does light regulate plant regeneration?. In Frontiers in Plant Science (Vol. 15). Frontiers Media SA. https://doi.org/10.3389/fpls.2024.1474431

Haring, F., Farid, M., Ridwan, I., Fadhilah, A. N., & Sedayu, N. (2024). Concentration of 2,4-D and BAP Combination on Callus Induction of Porang Plant (Amorphophallus muelleri Blume). Asian Journal of Plant Sciences, 23(3), 313–320. https://doi.org/10.3923/ajps.2024.313.320

Karimuna, S. R., Sulistiono, W., Taryono, Alam, T., & Wahab, A. (2023). Agronomic traits determinants of superior varieties and millable cane productivity of sugarcane (Saccharum officinarum L.) on dryland, Indonesia. Agronomy Research, 21(Special Issue 1), 306–319. https://doi.org/10.15159/AR.23.032

Kruglova, N., Zinatullina, A., & Yegorova, N. (2023). Histological Approach to the Study of Morphogenesis in Callus Cultures In Vitro: A Review. International Journal of Plant Biology 14(2), 533–545. MDPI. https://doi.org/10.3390/ijpb14020042

Manurung, B. H., Damanik, R. I., & Bayu, E. S. (2018). The Combination 2, 4-D and BAP to Induce Embryogenic Callus from Some Varieties of Soybean (Glycine max (L.) Merrill) on Hypoxic and In Vitro Condition. Jurnal Agroteknologi, 6(1), 86-92. https://doi.org/10.32734/ja.v6i1.2544

Meshram, J. H., Singh, S. B., Raghavendra, K. P., & Waghmare, V. N. (2022). Drought stress tolerance in cotton: progress and perspectives. Climate Change and Crop Stress: Molecules to Ecosystems, 135–169. https://doi.org/10.1016/B978-0-12-816091-6.00005-5

Munira, A., Bakhtiar, B., & Kesumawati, E. (2022). The Effect of Photoperiod and Auxins Plant Growth Regulators (2,4-D and IAA) to Callus Induction of Patchouli Leaves (Pogostemon cablin Benth.) under In Vitro Condition. Jurnal Agrista, 26(2).

Phamontree, S., Taratima, W., Songsri, P., & Jongrungklang, N. (2022). Development of a Highly Efficient Callus Induction and Regeneration Protocol for Sugarcane using Apical Meristem. Asian Journal of Plant Sciences, 21(1), 78–87. https://doi.org/10.3923/ajps.2022.78.87

Rahayu, S., Saptadi, D., Azmi, C., Kusumanegara, K., Handayani, T., Roostika, I., Bermawie, N., & Maulana, H. (2023). Callus Induction and Proliferation of Centella asiatica L. Generated from Leaves and Petioles in The Presence of Dicamba and BAP. Kultivasi, 22(3). https://doi.org/10.24198/kultivasi.v22i3.50581

Riajaya, P. D., Hariyono, B., Cholid, M., Kadarwati, F. T., Santoso, B., Djumali, & Subiyakto. (2022). Growth and Yield Potential of New Sugarcane Varieties during Plant and First Ratoon Crops. Sustainability (Switzerland), 14(21). https://doi.org/10.3390/su142114396

Ruíz-Rivas, M., Téllez-Valerio, C. E., Martínez-Núñez, M., Fernando Vera-Hernández, P., Martínez-Romero, E., & De Fátima Rosas-Cárdenas, F. (2022). Influence of light on callus generation and in vitro plant culture. Rev. Mex. Cienc. Agríc. esp. pub. Num, 13(27), 11-21. https://doi.org/10.29312/remexca.v13i27.3156

Sakadzo, N., Mubvuma, M., Mukanga, C., Mabveni, A. R. S., & Musundire, R. (2024). Phytochemical profiling of metabolites in commercial sugarcane (Saccharum officinarum L.) varieties that confer resistance to feeding of yellow sugarcane aphid (YSA) (Sipha flava) by using gas chromatography Mass Spectrometry (GC-MS) metabolomics approach. International Journal of Tropical Insect Science, 44(6), 2833–2847. https://doi.org/10.1007/s42690-024-01383-6

Setiawati, T., Arofah, A. N., & Nurzaman, M. (2020). Callus Induction of Chrysanthemum (*Chrysanthemum morifolium* Ramat. var. Tomohon Kuning) Using 2,4-Dichlorophenoxyacetic Acid (2,4-D) and 6-Benzylaminopurine (BAP) Under Different Light Conditions. Jurnal Pro-Life, 7(1), 13-26. https://doi.org/10.33541/pro-life.v7i1.1541

Setiawati, T., Astuti, A. L., Nurzaman, M., & Ratningsih, N. (2021). Analysis of Growth and Total Flavonoid Content in Chrysanthemum (*Chrysanthemum morifolium* Ramat) Callus Cultures Treated with 2,4-Dichlorophenoxyacetic Acid (2,4-D) and Coconut Water. Pro-Life, 8(1), 32-44. https://doi.org/10.33541/pro-life.v8i1.2781

Suhartanto, B., Astutik, M., Umami, N., Suseno, N., & Haq, M. S. (2022). The effect of explants and light conditions on callus induction of srikandi putih maize (Zea mays L.). IOP Conference Series: Earth and Environmental Science, 1001(1). https://doi.org/10.1088/1755-1315/1001/1/012006

Syabana, M. A., Marianingsih, P., Hermita, N., Rohimah, I. (2017). In Vitro Callus Induction and Growth of Stevia (Stevia rebaudiana Bertoni M.) with Difference Concentrations of PEG (Polyethylene Glycol) and Light Conditions. Jurnal Biodidaktika, 12(2). https://dx.doi.org/10.30870/biodidaktika.v12i2.2330

Thoriq, C. (2021). Teknik Budi Daya Tebu (1st Edition). Yogyakarta: DIVA Press.




DOI: https://doi.org/10.31764/justek.v9i3.40673

Refbacks

  • There are currently no refbacks.


JUSTEK : Jurnal Sains dan Teknologi sudah terindeks

EDITORIAL OFFICE: