Uji Aktivitas Ekstrak Etanol 70% Daun Ketapang (Terminalia catappa L.) Pada Hamster Syrian Jantan Hiperglikemia Dan Hiperkolesterolemia Dengan Parameter Pengukuran Kolesterol Total Dan LDL

Maharadingga Maharadingga, Ani Pahriyani, Desilva Arista

Abstract


ABSTRAK

Daun ketapang mengandung beberapa senyawa aktif seperti flavonoid, saponin, tanin, alkaloid, fenol dan triterpen. Senyawa flavonoid adalah salah satu senyawa polifenol yang memiliki efek antihiperkolesterolemia. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol 70% daun ketapang (Terminalia catappa L.) dalam menurunkan kadar kolesterol total dan LDL darah pada hamster syrian jantan hiperkolesterolemia dan hiperglikemia. Hewan uji dibagi 6 kelompok perlakuan, masing-masing kelompok terdiri dari 4 ekor hamster, yaitu kontrol normal, kontrol positif (atorvastatin), kontrol negatif, Kontrol Uji (KU) I, II, dan III. Pemberian ekstrak dan atorvastatin selama 14 hari setelah induksi pakan tinggi lemak dan aloksan. Hasil uji ANOVA satu arah menunjukkan adanya perbedaan setelah perlakuan. Selanjutnya dilakukan uji Tukey yang menunjukkan KU III (Dosis 120 mg/Kg BB) memiliki persentase terbesar dalam penurunan kadar kolesterol total dan LDL, namun tidak sebanding dengan kontrol positif. Kesimpulan, ekstrak etanol 70% daun ketapang aktif dalam menurunkan kolesterol total dan LDL namun tidak lebih baik dari atorvastatin

 

Kata kunci : Daun ketapang; Hiperglikemia; Hiperkolesterolemia;

Kolesterol total; LDL.


ABSTRACT

Ketapang leaves contain amount of active compounds such flavonoids, saponins, tannins, alkaloids, phenols and triterpenes. Flavonoids are polyphenolic compounds that have an anti-hypercholesterolemia effect. This study aimed to find the activities of 70% ketapang leaf (Terminalia catappa L.) ethanolic extract in reducing level of  LDL and total cholesterol in male Syrian hypercholesterolemia and hyperglycemia hamsters. Animals was devided into 6 treatment groups, each group consisted of 4 hamsters, namely normal control, positive control (atorvastatin), negative control, Test Control (TC) I, II, and III. Treatment was done by giving extracts and atorvastatin for 14 days after induction of high-fat and alloxan feed. The results of the one-way ANOVA test showed differences after treatment. Then the Tukey test has performed which showed that TC III (Dose of 120 mg/Kg BB) had the largest percentage in decreasing total cholesterol and LDL levels, but not comparable with positive controls. In conclusion, 70% ketapang leaf ethanolic extracts was active in reducing total cholesterol and LDL level but were not higher than atorvastatin.


Keyword: Ketapang leaf; Hyperglycemia; Hypercholesterolemia Total cholesterol; LDL.



Keywords


Ketapang leaf; Hyperglycemia; Hypercholesterolemia Total cholesterol; LDL.

Full Text:

PDF

References


Dachriyanus, Delpa OK, Rika O, Olivia E, Suhatri, M. Husni M. (2007). Uji Efek A-Mangostin terhadap Kadar Kolesterol Total, Trigliserida, Kolesterol HDL, dan Kolesterol LDL Darah Mencit Putih Jantan serta Penentuan Lethal Dosis 50 (Ld 50). Jurnal Sains dan Teknologi Farmasi. 12 (2) : 65

Departemen Kesehatan RI. (1985). Cara Pembuatan Simplisia. Depkes RI. Jakarta. Hlm. 7-15.

Departemen Kesehatan RI. (2008). Farmakope Herbal Indonesia. Direktorat Jendral Pengawasan Obat dan Makanan. Jakarta. Hlm. XXV, 169.

Divya N, Vijaya Anand A. phytochemical investigation and in vitro anti-diabetic activity of Terminalia catappa leaves. Int J Phyto Pharm. 2014;4:132–4.

Dwisari F, Harlia, Andi HA. (2016). Isolasi dan Karakterisasi Senyawa Terpenoid Ekstrak Metanol Akar Pohon Kayu Buta-buta (Excoecaria agallocha L.). JKK. Universitas Tanjungpura. 5 (3) : 26.

Egbung GE, Essien EU, Itam EH, Onouha AR. (2010). The Effect Saponin Consumption on Cholesterol Metabolism in Wistar Albino Rats. Research Journal of Agriculture and Biological Sciences. 6 (6) : 1071-1073.

Federer, W. (1963). Experimental Design Theory and Application. Oxford: Oxford and Lbh Publish Hinco.

Guyton AC, Hall JE. (2008). Buku Ajar Fisiologi Kedokteran. Edisi 11. EGC. Jakarta. Hlm. 324-375.

Hanani E. (2015). Analisis Fitokimia. Buku Kedokteran EGC. Jakarta. Hlm. 10, 11, 13, 75, 87, 114, 152, 236.

Istiqomah S. (2016). Pengaruh Ekstrak Daun Ketapang (Terminalia catappa L.) Terhadap Berat Badan dan Kadar Glukosa Darah Puasa Mencit (Mus musculus) Diabetik. Skripsi. Fakultas Sains dan Teknologi Universitas Airlangga. Surabaya.

Katzung BG. (2012). Farmakologi Dasar dan Klinik Vol.2. Edisi 12. EGC. Jakarta.

Kinoshita, Inoue Y, Nakama S, Ichiba T, Aniya Y. Antioxidant and hepatoprotective actions of medicinal herb, Terminalia catappa L. from Okinawa Island and its tannin corilagin. Phytomedicine Journal.. 2006;14(11):755–62. 12.

Kumari M, Jain S. (2012). Tannins: An Antinutrient With Positive Effect to Manage Diabetes. Research Journal of Recent Sciences. 1 (2) : 70-73.

Metwally MAA, El-Gellal AM, El-Sawaisi SM. (2009). Effects of Silymarin on Lipid Metabolism In Rats. World Applied Sciences Journal 6 (12) : 1634-1637.

Pirade PF. (2015). Perbandingan Pengaruh Anestesi Ketamin-Xylazin dan Ketamin-Zoletil Terhadap Fisiologi Kucing Lokal (Felis domestica). Skripsi. Program Studi Kedokteran Hewan. Fakultas Kedokteran Universitas Hasanuddin. Makassar.

Purwantini NM. (2015). Uji Aktifitas Antihiperkolesterol Ekstrak Etanol 70% Tempe Kacang Hijau pada Hamster Kolesterolemia Berdasarkan Kadar LDL dan Kolesterol Total. Skripsi. Fakultas Farmasi dan Sains Universitas Muhammadiyah Prof.DR.Hamka. Jakarta. Hlm. 14-15.

Priyanto. (2009). Farmakoterapi dan Teknologi Medis. Leskonfi. Depok. Hlm. 165.

Priyatno D. (2010). Paham Analisa Statistik Data dengan SPSS. MediaKom. Yogyakarta. Hlm. 71-76.

Reagan-Shaw S, Nihal M, Ahmad N. (2007). Dose Translation from Animal to Human Studies Revisited. The FASEB Journal. 22: 660.

Rowe CR, Sheskey PJ, Quinn ME. (2009). Handbook of Pharmaceutical Excipients Sixth Edition. Pharmaceutical Press and American Pharmacists Association. USA. Hlm. 119.

Suhono B. (2010). Ensiklopedia Flora Jilid V. PT.Kharisma Ilmu. Bogor. Hlm. 152

Szukudelski T. (2001). The Mechanism of Alloxan and Streptozotocin Action in B Cells of the Rat Pancreas. Journal of Physiological Research. Vol. 50. Hlm. 538.

Uttra KM, Bikha RD, Syed ZAS, Tarachand D, Thanwar D, Samar R. Lipid Profile of Patients With Diabetes Mellitus (A Multidisciplinary Study). World Applied Sciences Journal. 12 (9).

Vogel HG. (2008). Drug Discovery and Evaluation Pharmacological. Springer. USA. Hlm. 1674.

Waluyo Joko, Dwi Wahyuni. (2017). The Effect of Ketapang Leaf Extracts (Terminalia catappa L.) on The Cholesterol Levels of Male Mice (Mus musculus L.) Hypercholesterolemia. International Journal of Advanced Engineering Research and Science. 4 (7) : 45-48.




DOI: https://doi.org/10.31764/lf.v2i2.5488

Refbacks

  • There are currently no refbacks.


EDITORIAL OFFICE: